SEDFIT–MSTAR: molecular weight and molecular weight distribution analysis of polymers by sedimentation equilibrium in the ultracentrifuge

Determination of the Molecular Weight Distribution of Chondroitin Sulfate B by Sedimentation Equilibrium.

The discovery that purified protein preparations consist of molecules which are monodisperse with respect to molecular weight was originally entirely unexpected (1). Polymerization processes in general are expected to yield products which contain molecules with a wide range of molecular size, and only a template mechanism of synthesis can reasonably be expected to yield size homogeneity. It is ...

On the Calculation of Moments of Molecular Weight Distribution from Sedimentation Equilibrium Data

In this paper we discuss a technique for calculating moments of polydisperse materials in terms of concentration readings along the cell. The proposed method minimizes dependence on data from the en'd points ·where they may be unreliable'. An analysis is given of the errors involved in the use of the proposed method when the underlying molecular weight distribution is the Schulz distribution or...

The determination of density and molecular weight distributions of lipoproteins by sedimentation equilibrium.

A method is presented by which an experimental record of total concentration as a function of radial distance, obtained in a sedimentation equilibrium experiment conducted with a noninteracting mixture in the absence of a density gradient, may be analyzed to obtain the unimodal distributions of molecular weight and of partial molar volume when these vary concomitantly and continuously. Particul...

Rheological Method for Determining Molecular Weight and Molecular Weight Distribution

An Air-driven Ultracentrifuge for Molecular Sedimentation

Details of construction are given for an air-driven ultracentrifuge for molecular sedimentation. This instrument, like the standard oil-driven machine of Svedberg, uses rotors giving a 6.50 cm. radius of rotation and has cameras of great depth of focus.